Histology and Imaging Expertise
The Histology and Imaging Core is a centralized resource of expertise, equipment and technical capabilities. We support histological examination of tissues and cells, and provide access to advanced microscopy-based techniques and analysis for complete end-to-end analysis of biological samples (tissues to images/data).
The Histology and Imaging Core is supported by the Center for Cancer Biology Research CoBRE (NIGMS CoBRE P20GM103548).
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Services
Services we provide:
- Histology services for fresh, fixed or frozen tissue samples
- Processing
- Embedding
- Sectioning frozen or embedded tissue
- Staining techniques o Hematoxylin/Eosin (H&E) staining
- Immunohistochemistry (including antibody optimization)
- Special stains
- Light microscopy services (conventional and confocal)
- Training on various imaging techniques and analysis
- Assistance in acquiring live and fixed cell images
- Technical assistance when using specialized imaging procedures (FRET, TIRF, etc.)
- Automated whole slide/section imaging (brightfield/fluorescence)
- Consultation on imaging projects
- Troubleshooting imaging issues
Equipment
- Leica ASP300 Tissue Processor
- Sakura Tissue-Tek III Embedder
- Ventana Benchmark XT Tissue Stainer
- Nikon A1R Confocal Microscope
- Nikon A1 (with TIRF) Confocal Microscope
- Nikon-CSU-W1 SoRa Super Resolution Microscope
- Olympus FV-1000 Confocal Microscope
- Nikon Ni-E microscope (x2)
- Olympus BX-51 microscope
- Olympus IX-71 microscope
- Nikon SMZ-1000 Stereomicroscope
- StereoInvestigator System
Publications
Roux KJ, Kim DI, Burke B, May DG. BioID: A Screen for Protein-Protein Interactions. Current Protocols in Protein Science. 91:19.23.1-19.23.15., 2018. PMID29516480.
KC B, May DG, Benson BV, Kim DI, Shivega WG, Ali MH, Faustino RS, Campos AR and Roux KJ. VRK2A is an A-type lamin-dependent nuclear envelope kinase that phosphorylates BAF. Mol Biol Cell. 2017 Aug 15; 28(17): 2241-2250.
Messerli SM, Hoffman MM, Gnimpieba EZ, Bhardwaj RD. Therapeutic Targeting of PTK7 is Cytotoxic in Atypical Teratoid Rhabdoid Tumors. Mol Cancer Res. 2017 Apr 25; PubMed PMID: 28442586.
Messerli S M, Hoffman MM, Gnimpieba EZ, Kohlhof H, and Bhardwaj RD. 4SC-202 as a potential treatment for the pediatric tumor medulloblastoma. Brain Sci. 2017, 7, 147; doi:10.3390/brainsci7110147.
Otterpohl KL, Hart RG, Evans C, Surendran K, and Chandrasekar I. Nonmuscle myosin 2 proteins encoded by Myh9, Myh10, and Myh14 are uniquely distributed in the tubular segments of murine kidney. Physiol Rep. 2017 Dec;5(23). pii: e13513. doi: 10.14814/phy2.13513.
Louwagie EJ, Larsen TD, Wachal AL, and Baack ML. Placental lipid processing in response to a maternal high-fat diet and diabetes in rats. Pediatr Res. 2017. Epub 2018 Jan 3. Print 2018 Mar;83(3):712-722. doi: 10.1038/pr.2017.288.
Anderson RH and Francis KR. Modeling rare diseases with induced pluripotent stem cell technology. Molecular and Cellular Probes 2018 Aug; 40:52-59. doi: 10.1016/j.mcp.2018.01.001. Epub 2018 Jan 5. PMID: 29307697.
Brudvig, JJ, Cain, JT, Sears, RM, Schmidt-Grimminger, GG, Wittchen, ES, and Weimer, JM. MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network. Scientific Reports. (2018).
Madeo, M, Colbert, PL, Vermeer, DW, Lucido, CT, Cain, JT, Vichaya, EG, Grossberg, AJ, Muirhead, D, Rickel AP, Hong, Z, Zhao, J, Weimer, JM, Spanos, WC, Lee, JH, Dantzer, R, and Vermeer, PD. Cancer exosomes induce tumor innervation. Nature Communication. (2018).
Brudvig, JJ, Cain, JT, Schmidt-Grimminger, GG, Stumpo, DJ, Roux, KJ, Blackshear, PJ, and Weimer, JM (2018) MARCKS is necessary for Netrin-DCC signaling and corpus callosum formation.
Molecular Neurobiology. [Epub ahead of print]. PMID:29546593.
Lucido CT, Wynja E, Madeo M, Schwartz LE, Drapkin R, Vermeer, PD. Cervical cancer-derived exosomes induce neurite outgrowth. Gynecologic Oncology Apr 16. pii: S0090-8258(19)31149-7. doi: 10.1016/j.ygyno.2019.04.651 (2019) [epub ahead of print].Vermeer PD. Exosome induction of tumor innervation. Invited review, Cancer Research, in press.
Lucido, CT, Miskimins, WK, Vermeer PD. Propranolol promotes glucose dependence and synergizes with dichloroacetate for anti-cancer activity in HNSCC. Cancers 10: 476 (2018).
Lucido, CT, Callejas-Valera JL, Colbert, PL, Vermeer, DW, Miskimins, WK, Spanos, WC, and Vermeer, PD. Β2-Adrenergic receptor modulates mitochondrial metabolism and disease progression in recurrent/metastatic HPV(+) HNSCC. Oncogenesis 7: 81 (2018).
Madeo M, Colbert PL, Vermeer DW, Lucido CT, Vichaya EG, Grossberg AJ, Cain JT, Muirhead D, Rickel AP, Hong Z, Spanos WC, Lee JH, Dantzer R, Vermeer PD. Cancer exosomes induce tumor neo-neurogenesis potentiating tumor growth Biorxiv2018 January. Available from: biorxiv.org/content/early/2018/01/12/247452
Vermeer DW, Coppock JD, Zeng E, Lee KM, Spanos WC, Onken MD, Uppaluri R, Lee JH, Vermeer PD. Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis. Oncotarget 7: 24228-24241 (2016).
Colbert PL, Vermeer DW, Wieking BG, Lee JH, Vermeer PD. EphrinB1: novel microtubule associated protein whose expression affects taxane sensitivity. Oncotarget 6(2):953-68 (2014).
Lucido, CT, Vermeer PD, Wieking BG, Vermeer DW, Lee JH. CD137 Enhancement of HPV Positive Head and Neck Squamous Cell Carcinoma Tumor Clearance. Vaccines 2: 841-853 (2014).