Histology and Imaging Expertise
The Histology and Imaging Core is a centralized resource of expertise, equipment and technical capabilities. We support histological examination of tissues and cells, and provide access to advanced microscopy-based techniques and analysis for complete end-to-end analysis of biological samples (tissues to images/data).
The Histology and Imaging Core is supported by the Center for Cancer Biology Research CoBRE (NIGMS CoBRE P20GM103548).
Services we provide:
- Histology services for fresh, fixed or frozen tissue samples
- Sectioning frozen or embedded tissue
- Staining techniques o Hematoxylin/Eosin (H&E) staining
- Immunohistochemistry (including antibody optimization)
- Special stains
- Light microscopy services (conventional and confocal)
- Training on various imaging techniques and analysis
- Assistance in acquiring live and fixed cell images
- Technical assistance when using specialized imaging procedures (FRET, TIRF, etc.)
- Automated whole slide/section imaging (brightfield/fluorescence)
- Consultation on imaging projects
- Troubleshooting imaging issues
- Leica ASP300 Tissue Processor
- Sakura Tissue-Tek III Embedder
- Ventana Benchmark XT Tissue Stainer
- Nikon A1R Confocal Microscope
- Nikon A1 (with TIRF) Confocal Microscope
- Olympus FV-1000 Confocal Microscope
- Nikon Ni-E microscope (x2)
- Olympus BX-51 microscope
- Olympus IX-71 microscope
- Nikon SMZ-1000 Stereomicroscope
- StereoInvestigator System
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Madeo, M, Colbert, PL, Vermeer, DW, Lucido, CT, Cain, JT, Vichaya, EG, Grossberg, AJ, Muirhead, D, Rickel AP, Hong, Z, Zhao, J, Weimer, JM, Spanos, WC, Lee, JH, Dantzer, R, and Vermeer, PD. Cancer exosomes induce tumor innervation. Nature Communication. (2018).
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Molecular Neurobiology. [Epub ahead of print]. PMID:29546593.Lucido CT, Wynja E, Madeo M, Schwartz LE, Drapkin R, Vermeer, PD. Cervical cancer-derived exosomes induce neurite outgrowth. Gynecologic Oncology Apr 16. pii: S0090-8258(19)31149-7. doi: 10.1016/j.ygyno.2019.04.651 (2019) [epub ahead of print].
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